Metabolic changes in mouse sperm during capacitation†.

TitleMetabolic changes in mouse sperm during capacitation†.
Publication TypeJournal Article
Year of Publication2020
AuthorsBalbach M, Gervasi MGracia, Hidalgo DMartin, Visconti PE, Levin LR, Buck J
JournalBiol Reprod
Volume103
Issue4
Pagination791-801
Date Published2020 Oct 05
ISSN1529-7268
Abstract

Mammalian sperm are stored in the epididymis in a dormant state. Upon ejaculation, they must immediately start producing sufficient energy to maintain motility and support capacitation. While this increased energy demand during capacitation is well established, it remains unclear how mouse sperm modify their metabolism to meet this need. We now show that capacitating mouse sperm enhance glucose uptake, identifying glucose uptake as a functional marker of capacitation. Using an extracellular flux analyzer, we show that glycolysis and oxidative phosphorylation increase during capacitation. Furthermore, this increase in oxidative phosphorylation is dependent on glycolysis, providing experimental evidence for a link between glycolysis and oxidative phosphorylation in mouse sperm.

DOI10.1093/biolre/ioaa114
Alternate JournalBiol Reprod
PubMed ID32614044